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In my case the amount of dna for library preparation is low but to a specific gene.
Chip seq library preparation. Truseq chip library preparation kits are compatible with all illumina sequencers. Please note that adaptors and primers are not included in the kit and are available separately. What quantity of chip dna is recommended for library preparation. The chip seq library prep reagent set for illumina contains enzymes and buffers that are ideally suited for sample preparation for next generation sequencing and for preparation of expression libraries.
The nebnext chip seq library prep master mix set for illumina contains reagents for preparation of libraries for next generation sequencing on the illumina platform from 10 ng of chip dna in a standard workflow. Preparation methods for chomatin immunoprecipitated chip seq dna libraries are similar to those for standard dna. Input dna amount from 5 ng to 30 ng. If you have less than 1 ng consider pooling replicate chip samples together in a maximum volume of 30 µl.
However the amount of input dna for chip seq libraries is often lower than for standard dna library construction. In the regular chip seq although 10ng dna is a lot to a specific gene the amoount of dna is still very low. High library conversion efficiency. Simple hands on time 10 min.
Offering the proven data quality and ease of use of truseq dna sequencing they provide a streamlined chip seq library preparation workflow that leverages reagent master mixes to minimize pipetting and reduce total assay time. Chip seq library prep fast total time 1 5 hrs. Bioo scientific nextflex chip for the illumina platform. A quantity of 1 10 ng of chip dna is recommended for constructing a library with the nebnext chip seq library prep kit.
Preparation methods for chromatin immunoprecipitation chip seq dna libraries are similar to those for standard dna.
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